View

BBa_I12023

BBa_I12023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I12023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ryhsiao

Date created: 2004-07-25 11:00:00

Date modified: 2015-08-31 04:07:31

Inducible Lambda cI Repressor Generator (Controlled by ATC and TetR)

Back

Details

Types	DnaRegion
Roles	engineered_region Generator
Sequences	BBa_I12023_sequence (Version 1)

Description

This part will produce Lambda cI Repressor when ATC is introduced into the system. The system must also be free of p22 cII for Lambda cI repressor to be expressed.

Notes

The absence of p22 cII (constitutive promoter) will yield a constant promotion of TetR, which inhibits the expression of lambda cI. Once ATC is introduced into the system, it will bind to the TetR, resulting in expression/promotion of lambda cI repressor via the QPI (Quad Part Inverter). Hence, a switch controlled by ATC is created.

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0053 BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012	cII p22 BBa_B0034 tetR BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 cI lam BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0053 BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012	1,54 63,74 81,740 774,853 862,902 911,964 973,984 991,1740 1774,1853 1862,1902	cII p22 BBa_B0034 tetR BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 cI lam BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_I12023/1