| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_I12031_sequence (Version 1)
|
Description
This forms the activator of a Barkai-Leibler oscillator. Differs from BBa_I12000 in that this part includes an extra 434 OR region downstream of the -10 site to allow the repression to take advantage of cooperativity.
Notes
This part to work in conjunction with BBa_I12002. This part creates one of the proteins involved in the Modified Barkai-Leibler relaxation oscillator design (activator protein: lambda cI). In the presence of Lambda cI, more Lambda cI will be produced (positively autoregulated). However, if there is 434 cI repressor present in the system, the production of Lambda cI should be repressed. This was implemented in the subpart BBa_I12030, which represents the Operating Regions for the promoter. The fact that the OR-3 site was replaced with the OR-1 site of 434 cI, and that the OR-2 site of 434 cI is included directly after the -10 site should provide enough cooperativity/binding affinity for the lambda cI to be sufficiently repressed when there is 434 cI binding in.
Source
Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
