Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Kelly, Karen Wong, Nishant Bhat
Date created: 2007-08-14 11:00:00
Date modified: 2015-08-31 04:07:39
Device used to characterize Ribosome Binding Sites through measurement of GFP expression
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_I20248_sequence (Version 1) |
Description
Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction.Notes
.Source
.| Sequence Annotation | Location | Component / Role(s) |
| TetR 1 BBa_R0040 -35 TetR 2 -10 GFP protein BBa_E0040 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,19 1,54 20,25 26,44 43,48 61,780 61,780 789,868 800,843 877,917 884,903 904,917 910,910 | non_covalent_binding_site feature/binding feature/BioBrick engineered_region feature/promoter promoter feature/binding non_covalent_binding_site feature/promoter promoter feature/protein CDS engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon |