Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Kelly, Karen Wong, Nishant Bhat
Date created: 2007-08-14 11:00:00
Date modified: 2015-08-31 04:07:39
Device used to characterize Ribosome Binding Sites through measurement of GFP expression
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_I20249_sequence (Version 1) |
Description
Device is based on I2055 and contains a partial biobrick site (Spe1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter Xba1 and Spe1 and the kit EcoR1 and Xba1. Use .5ul of each restriction enzyme during the ligation to ensure the formation of two mixed sites. This design provides the proper spacing, identical to actual construction conditions.Notes
.Source
.| Sequence Annotation | Location | Component / Role(s) |
| TetR 1 BBa_R0040 -35 TetR 2 -10 GFP protein BBa_E0040 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,19 1,54 20,25 26,44 43,48 61,780 61,780 789,868 800,843 877,917 884,903 904,917 910,910 | feature/binding non_covalent_binding_site engineered_region feature/BioBrick promoter feature/promoter feature/binding non_covalent_binding_site promoter feature/promoter CDS feature/protein feature/BioBrick engineered_region engineered_region feature/BioBrick feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop feature/polya polyA_site stop_codon feature/stop |