Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Kelly, Karen Wong, Nishant Bhat
Date created: 2007-08-14 11:00:00
Date modified: 2015-08-31 04:07:39
Device used to characterize Ribosome Binding Sites through measurement of RFP expression
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_I20251_sequence (Version 1) |
Description
Device is based on I2056 and contains a partial biobrick site (Spe1, Not1, Xba1) between R0040 and RFP. To insert a BB promoter for testing, digest the promoter Xba1 and Spe1 and the kit EcoR1 and Xba1. Use .5ul of each restriction enzyme during the ligation to ensure the formation of two mixed sites. This design provides the proper spacing, identical to actual construction conditions.Notes
.Source
.| Sequence Annotation | Location | Component / Role(s) |
| TetR 1 BBa_R0040 -35 TetR 2 -10 mrfp1 BBa_E1010 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,19 1,54 20,25 26,44 43,48 61,735 61,741 742,766 775,854 786,829 863,903 870,889 890,903 896,896 | feature/binding non_covalent_binding_site feature/BioBrick engineered_region feature/promoter promoter non_covalent_binding_site feature/binding promoter feature/promoter feature/cds CDS engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya feature/stop stop_codon |