BBa_I3521

BBa_I3521 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I3521

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)

Date created: 2004-01-28 12:00:00

Date modified: 2015-08-31 04:07:41

Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500)

Details

• Types: DnaRegion
• Roles: Project; engineered_region
• Sequences: BBa_I3521_sequence (Version 1)

Description

Tests cellular density of asparate-secreting cells needed to induce quorum threshold response. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells. Quorum threshold is detected when the LacZ reporter is observed.

Notes

Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli. This test combines the chemotaxis response test with the quorum threshold test.

Components

Access	Instance	Definition
public	BBa_I3600	BBa_I3600
public	BBa_I3520	BBa_I3520
public	BBa_I3500	BBa_I3500

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I3600	1,4067	BBa_I3600
BBa_I3520	4076,5729	BBa_I3520
BBa_I3500	5738,6598	BBa_I3500

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I3521/1

Member of these Collections

• //classic/project/uncategorized
• iGEM Parts Registry