Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-28 12:00:00
Date modified: 2015-08-31 04:07:41
Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422)
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_I3600_sequence (Version 1) |
Description
Tests the chemotaxis cell-cell signalling machinery based on aspartate secretions. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells.Notes
Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli.| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_R1075 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0034 BBa_C0083 BBa_B0034 BBa_E0022 BBa_B0010 BBa_B0012 | const. BBa_B0034 lacI BBa_B0010 BBa_B0012 LacI BBa_B0034 aspA BBa_B0034 ECFP BBa_B0010 BBa_B0012 |