View

BBa_I3601

BBa_I3601 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I3601

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)

Date created: 2004-01-28 12:00:00

Date modified: 2015-08-31 04:07:41

Test: Chemotaxis response to secreted aspartate, receiver cells (R1075.E0432)

Back

Details

Types	DnaRegion
Roles	engineered_region Project
Sequences	BBa_I3601_sequence (Version 1)

Description

Tests the chemotaxis cell-cell signalling machinery based on aspartate secretions. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells.

Notes

Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli.

Components

Access	Instance	Definition
public public public public public	BBa_R1075 BBa_B0034 BBa_E0032 BBa_B0010 BBa_B0012	const. BBa_B0034 YFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R1075 BBa_B0034 BBa_E0032 BBa_B0010 BBa_B0012	1,49 58,69 76,837 846,925 934,974	const. BBa_B0034 YFP BBa_B0010 BBa_B0012