pSC101

BBa_I50041 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I50041
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2006-03-13 12:00:00
Date modified: 2015-08-31 04:07:42

pSC101 origin of replication, reverse



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_I50041_sequence (Version 1)

Description

pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040.

Sequence Annotation Location Component / Role(s)
putative polyA
repA
inverted repeat
RBS
RBS
promoter region
inverted repeat
rep promoter -10
rep promoter -35
Mutated SpeI site
direct repeat
direct repeat
direct repeat
AT rich region
par region
inverted repeat
inverted repeat
inverted repeat
Tn3 integration site
inverted repeat
terminator
difference from canonical pSC101 sequence in Genbank
difference from canonical pSC101 sequence in Genbank
difference from canonical pSC101 sequence in Genbank
difference from canonical pSC101 sequence in Genbank
76,91
202,1152
1154,1176
1154,1159
1156,1168
1173,1192
1179,1200
1183,1188
1205,1210
1219,1224
1250,1269
1282,1301
1303,1322
1321,1403
1534,1901
1800,1817
1823,1846
1857,1873
1876,1880
1886,1899
1963,1992
36,36
126,126
288,288
383,383
feature/polya polyA_site
feature/cds CDS
feature/misc sequence_feature
feature/rbs ribosome_entry_site
ribosome_entry_site feature/rbs
promoter feature/promoter
sequence_feature feature/misc
feature/promoter promoter
promoter feature/promoter
sequence_alteration feature/mutation
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
feature/stem_loop stem_loop
sequence_alteration feature/mutation
sequence_alteration feature/mutation
sequence_alteration feature/mutation
feature/mutation sequence_alteration
igem#experience None  
igem#sampleStatus Not in stock  
igem#status Unavailable  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_I50041/1