BBa_I715034

BBa_I715034 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I715034

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrew Martens

Date created: 2007-07-19 11:00:00

Date modified: 2015-08-31 04:07:49

pLAC-RBS-RFP1-HixC-RFP2

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_I715034_sequence (Version 1)

Description

This part is designed to test whether or not RFP tolerates a HixC insertion. Cells with this part should turn red.

Notes

Note that, to avoid a frameshift, HixCRFP2 was designed such that it has an extra nucleotide between the HixC site and the RFP2 section.

Source

Ligated BBa_S03738 with BBa_S03740.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_I715022	RFP1
public	BBa_J44000	hixC
public	BBa_I715023	RFP2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_I715022	227,688	RFP1
BBa_J44000	697,722	hixC
BBa_I715023	731,950	RFP2

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I715034/1

Member of these Collections

• iGEM Parts Registry