CPX

BBa_I728500 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I728500

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Toan Tran-Phu

Date created: 2007-07-30 11:00:00

Date modified: 2015-08-31 04:07:55

CPX Terminal Surface Display Protein with Polystyrene-Binding Peptide

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_I728500_sequence (Version 1)

Description

This will be filled in later.

Notes

Max of XXXX amino acid long peptide.

Source

Rice JJ, Schohn A, Bessette PH, Boulware KT, and Daugherty PS. Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Sci 2006 Apr; 15(4) 825-36. doi:10.1110/ps.051897806 pmid:16600968

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start codon	1,3	start_codon, feature/start
Native Starting Signal	1,69	CDS, feature/protein
SfiI Restriction Site	70,84	feature/dna, sequence_feature
Polystyrene Sticky Peptide	91,129	feature/protein, CDS
Linker	175,192	feature/misc, sequence_feature
S54-F148 of OmpX	193,477	feature/protein, CDS
Linker	478,489	feature/misc, sequence_feature
A1-S53 of OmpX	490,648	CDS, feature/protein
Double stop codon (TGATAA)	649,654	stop_codon, feature/stop
BglII Cut Site	85,90	sequence_feature, feature/dna
T7 Antibody Tag	142,174	feature/tag, tag
SalI Restriction Site	130,135	feature/dna, sequence_feature
Trypsin Cleavage Site	136,141	feature/protein, CDS
Coding sequence	1,654	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_I728500/1

Member of these Collections

• //cds/membrane/receptor
• iGEM Parts Registry