| Types | DnaRegion
|
| Roles | Composite
engineered_region
|
| Sequences | BBa_I728978_sequence (Version 1)
|
Description
When transformed into escherichia coli, this part will result in the terminal display of a polystyrene-binding peptide that will increase e. coli's binding affinity to plastic. The peptide displayed may be switched with another using the restriction sites BgII and SalI flanking the polystyrene-binding peptide sequence.
Notes
We decided to use F2620 as a promoter because we weren't sure how many displayed copies of our engineered CPX could be handled by e. coli and F2620 allowed us to test multiple levels of transcription and translation of CPX to find the most effective rates.
Source
CPX sequence comes from Rice et. al "Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands", while the polystyrene-binding insert sequence orinated from Kenan et. al "Peptide-PEG Amphiphiles as Cytophobic Coatings for Mammalian and Bacterial Cells"
