BBa_I742113

BBa_I742113 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I742113

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: sarah hollingshead

Date created: 2007-10-18 11:00:00

Date modified: 2015-08-31 04:08:02

ech gene with ribosome binding site

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_I742113_sequence (Version 1)

Description

encodes enoyl CoA hydratase (cleaves CoA group from feruloyl CoA)

ech converts feruloyl CoA to vanillin

catalyses the final stage of the vanillin biosynthesis pathway

Notes

P. fluorescens has a high GC genome, therefore we had the coding region optimised for expression in E. coli.

Gene was synthesised by geneart

removed one PstI site

Source

Pseudomonas fluorescens NCIMB 9046

Gene was synthesised by geneart

Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)

Components

Access	Instance	Definition
public	BBa_J15001	BBa_J15001
public	BBa_I742112	BBa_I742112

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J15001	1,10	BBa_J15001
BBa_I742112	17,850	BBa_I742112

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I742113/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 17P
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 17P
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry