Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:02
B-carotene (orange pigment) producing operon
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_I742121_sequence (Version 1) |
Description
Part contains the four genes CrtEBIY, which form the B-carotene biosynthesis pathway.The four genes sequentially convert fanesyl diphosphate into B-carotene (a vitamin A precursor)
B-carotene also has an orange pigment
Notes
the crtI coding sequence contains 2 PstI sites. These have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed. This version still contains the two PstI sitesSource
Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005)Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321)
| Access | Instance | Definition |
| public public public | BBa_I742116 BBa_I742119 BBa_I742117 | BBa_I742116 BBa_I742119 BBa_I742117 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I742116 BBa_I742119 BBa_I742117 | 1,954 963,3392 3401,4571 | BBa_I742116 BBa_I742119 BBa_I742117 |