Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03
B-carotene (organge pigment) producing operon without PstI sites
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I742138_sequence (Version 1) |
Description
B-carotene is an intermeidate in the Zeaxanthin biosynthesis pathway.The four genes, CrtE, CrtB, CrtI and CrtY, encode four enzymes which sequentially convert farnesyl diphosphate (produced by the mevlonate pathway) into B-carotene.
More information on this biosynthesis pathway may be found on the Edinburgh Yoghurt Wiki
Notes
Two PstI sites were removed from the CrtI gene via point mutagenesisAll four genes were cloned so as to retain their native ribosome binding sites
Source
All four genes were isolated from Pantoea ananatis (formerly Erwinia uredovora)DSMZ 30080, ATCC 19321
Carotenoid Pathway references:
Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005)
Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
| Access | Instance | Definition |
| public public public | BBa_I742116 BBa_I742133 BBa_I742117 | BBa_I742116 BBa_I742133 BBa_I742117 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I742116 BBa_I742133 BBa_I742117 | 1,954 963,3392 3401,4571 | BBa_I742116 BBa_I742133 BBa_I742117 |