Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lovelace Soirez
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:04
I0500 (Inducible pBad/araC) + I746001 (AIP generator - agrB + agrD with RBSes + Terminator)
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_I746008_sequence (Version 1) |
Description
This part is the AIP generator from the agr system with an inducible promoter upstream (arabinose).pBad is an E. coli promoter that is tightly controlled by:
* inducer: L-arabinose.
* repressor: AraC apparently acts as the repressor
The original (I746000) part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. It contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.
In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
Notes
N/ASource
The original sequences for agrB and agrD were taken from the S. aureus Sequencing Project at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.| Access | Instance | Definition |
| public public public public | BBa_I0500 BBa_I746000 BBa_B0010 BBa_B0012 | pBad/araC BBa_I746000 BBa_B0010 BBa_B0012 |