Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lovelace Soirez
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:04
I746100 (AIP sensor infrastructure - agrC + agrA with RBSes) + B0015 (Terminator)
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_I746101_sequence (Version 1) |
Description
This part is simply the AIP sensor infrastructure (I746001) from the agr system with a standard registry terminator (B0015) downstream.The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.
In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.
Notes
N/ASource
The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.| Access | Instance | Definition |
| public public public | BBa_I746100 BBa_B0010 BBa_B0012 | BBa_I746100 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I746100 BBa_B0010 BBa_B0012 | 1,1920 1929,2008 2017,2057 | BBa_I746100 BBa_B0010 BBa_B0012 |