BBa_I746902

BBa_I746902 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I746902

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stefan Milde

Date created: 2008-09-30 11:00:00

Date modified: 2015-08-31 04:08:05

pbad promoter driving 6-his tagged mut3 GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_I746902_sequence (Version 1)

Description

This part is used for purification of mut3 GFP via its 6-his tag. It is driven by the arabinose - sensitive pbad promoter. Induction should be with 1mM arabinose for overnight growth of a 50ml (or more) culture for purification the next day.

Notes

no special considerations.

Source

The part was assembled from intermediates via standard assembly.

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_B0034	BBa_B0034
public	BBa_I746918	BBa_I746918
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_B0034	1219,1230	BBa_B0034
BBa_I746918	1237,1974	BBa_I746918
BBa_B0010	1983,2062	BBa_B0010
BBa_B0012	2071,2111	BBa_B0012

Other Properties

• igem#experience: Works
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I746902/1

Member of these Collections

• iGEM 2018 Cell E. coli strain NEB 10-beta
• iGEM 2018 Distribution Plate 4
• iGEM 2018 Distribution Plate 4 Well 13I
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry