Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Phillip Dodson
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:05
EcoRI-GenR-XbaI on pSB1AK3 backbone
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_I750017_sequence (Version 1) |
Description
This part was created from BBa_J61035 when it was cut with EcoRI and SpeI and this section inserted before a terminator BBa_B0015 on a pSB1AK3 plasmid.The part was never created in the pure form since when created it carried our part of interest between Xbal and SpeI.
Creating it allows multiple parts to be shipped as a parts in this plasmid without further complicating explanations.
This should perform a usefull service since many expression parts require the addition of RBS and terminator which is conveniently done using this sequence of events due to the multiple antibiotic resistances collected during the process.
Notes
noneSource
Part from kit provided BBa_J61035 and BBa_B0015.| Sequence Annotation | Location | Component / Role(s) |
| beta-lactamase (ampR) promoter gentamycin resistance marker R6Kg origin beta-lactamase ampicillin resistance marker kanamycin resistance pMB1 origin BioBrick suffix EcoRI NotI XbaI Verfication forward (VF2) primer binding site Stem loop End of stem loop E. coli his operon terminator Stem loop Deletion of AC Verification reverse (VR) primer binding site | 2969,2997 3645,4178 3201,3584 2067,2927 1097,1912 261,875 1,21 3168,3173 3624,3631 4643,4648 3050,3069 3139,3158 3126,3129 22,93 30,64 34,35 157,176 | feature/promoter promoter CDS feature/cds feature/dna sequence_feature CDS feature/cds CDS feature/cds feature/dna sequence_feature feature/dna sequence_feature sequence_feature feature/dna feature/dna sequence_feature feature/dna sequence_feature primer_binding_site feature/primer_binding feature/stem_loop stem_loop feature/stem_loop stem_loop feature/stem_loop stem_loop stem_loop feature/stem_loop feature/mutation sequence_alteration feature/primer_binding primer_binding_site |