Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: 2007 NYMU_Taiwan iGEM Team
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:08
Glucose controlled insulin generator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_I761005_sequence (Version 1) |
Description
This generator will response to extracellular glucose level. If the level of extracellular glucose is high, the generator will turn on; If the level of extracellular glucose is low, the gene will turn off. Further characterization is needed to determine the threshold extracellular glucose concentration.Notes
The A and B chain of human insulin are encoded separately because E. coli lack human post-translational modification mechanism. To ensure proper disulfide bond formation, this generator must be transformed into periplasmic oxidase mutant strain like DR473.Source
*BBa_B0034*BBa_R0082
*BBa_I761002
*BBa_I761003
| Access | Instance | Definition |
| public public public public public | BBa_R0082 BBa_B0034 BBa_I761002 BBa_B0034 BBa_I761003 | OmpR BBa_B0034 BBa_I761002 BBa_B0034 BBa_I761003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0082 BBa_B0034 BBa_I761002 BBa_B0034 BBa_I761003 | 1,108 117,128 135,285 294,305 312,497 | OmpR BBa_B0034 BBa_I761002 BBa_B0034 BBa_I761003 |