Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin McGuire
Date created: 2012-10-04 11:00:00
Date modified: 2015-08-31 04:08:22
E. coli optimized CDM
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_J100100_sequence (Version 1) |
Description
E. coli optimized codon encoding the caffeine demethylase developed by Christina Smolke lab. Converts caffeine into theophylline.Notes
Had to remove internal Pst1 sites.Source
Amino Acid Sequence taken from: Michener and Smolke. 2012. Metabolic Engineering.14. 306-316. This is the equivalent to yCDM8| Sequence Annotation | Location | Component / Role(s) |
| Bba_prefix Bba_suffix start Stop BsaI cuts right BsaI sticky end | 1,22 3184,3204 31,33 3178,3183 23,28 30,33 | feature/BioBrick engineered_region feature/BioBrick engineered_region start_codon feature/start feature/stop stop_codon feature/binding non_covalent_binding_site feature/misc sequence_feature |