BBa_J100100

BBa_J100100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin McGuire
Date created: 2012-10-04 11:00:00
Date modified: 2015-08-31 04:08:22

E. coli optimized CDM



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_J100100_sequence (Version 1)

    Description

    E. coli optimized codon encoding the caffeine demethylase developed by Christina Smolke lab. Converts caffeine into theophylline.

    Notes

    Had to remove internal Pst1 sites.

    Source

    Amino Acid Sequence taken from: Michener and Smolke. 2012. Metabolic Engineering.14. 306-316. This is the equivalent to yCDM8

    Sequence Annotation Location Component / Role(s)
    Bba_prefix
    Bba_suffix
    start
    Stop
    BsaI cuts right
    BsaI sticky end
    		1,22
    3184,3204
    31,33
    3178,3183
    23,28
    30,33
    		feature/BioBrick engineered_region
    feature/BioBrick engineered_region
    start_codon feature/start
    feature/stop stop_codon
    feature/binding non_covalent_binding_site
    feature/misc sequence_feature

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J100100/1
     

    Attachments

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