BBa_J100101

BBa_J100101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin McGuire

Date created: 2012-11-13 12:00:00

Date modified: 2015-08-31 04:08:22

High production of eCDM8

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_J100101_sequence (Version 1)

Description

High promotor and high RBS (c-Dog). E. coli optimized caffeine demethylase with the same amino acid sequence as yCDM8 from Michener and Smolke (2012).

Notes

S05008 was ligated using Bsa1, so there is no scar between the two parts and Pst1

Source

eCDM8 was synthesized de novo.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J119031	1,36	engineered_region, feature/BioBrick
BBa_J119025	37,124	engineered_region, feature/BioBrick
leader RBS	55,63	ribosome_entry_site, feature/rbs
Start	69,71	feature/start, start_codon
GOI RBS	107,115	sequence_feature, feature/misc
Stop	120,122	feature/stop, stop_codon
Start	122,124	start_codon, feature/start
BBa_J100100	125,3328	engineered_region, feature/BioBrick
BsaI sticky end	121,124	feature/misc, sequence_feature
Stop	3302,3307	stop_codon, feature/stop
Bba_suffix	3275,3295	engineered_region, feature/BioBrick
Double Stop	3269,3274	stop_codon, feature/stop

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100101/1

Member of these Collections

• iGEM Parts Registry