Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2013-04-17 11:00:00
Date modified: 2015-08-31 04:08:22
adhE optimized for E. coli, PstI removed
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_J100109_sequence (Version 1) |
Description
This CDS codes for the adhE gene from E. coli and can be used in conjunction with an adhE- strain for complementation. This has been designed to go downstream of a C-Dog RBS using golden gate assembly (GGA) with BsaI as the restriction enzyme.Notes
Had to remove 4 internal PstI sites. Codons were optimized for E. coli.Source
gi|49175990:1294669-1297344 Escherichia coli str. K-12 substr. MG1655 chromosome, complete genomeReverse strand.
| Sequence Annotation | Location | Component / Role(s) |
| suffix EcoRI site BsaI cuts right BsaI sticky end start adhE allele stop | 2692,2711 1,6 7,12 14,17 15,17 15,2687 2688,2690 | engineered_region feature/BioBrick feature/dna sequence_feature feature/dna sequence_feature feature/misc sequence_feature start_codon feature/start feature/cds CDS feature/stop stop_codon |