Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2013-09-05 11:00:00
Date modified: 2015-08-31 04:08:23
P5+riboswitch+adhE
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_J100122_sequence (Version 1) |
Description
This is a strong promoter driving transcription of theophylline riboswitch D followed by E. coli optimized adhE gene. Used in combination with adhE deletion strain SHH31.Notes
Built by Golden Gate Assembly.Source
built from stock parts in Registry.| Sequence Annotation | Location | Component / Role(s) |
| P5 Promoter lac operator scar constant sequence Knpl site Riboswitch RBS adhE allele deletion one codon (V) Stop | 1,53 54,75 76,81 83,97 98,103 104,163 147,150 161,2830 176,177 2831,2833 | promoter feature/promoter ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs CDS feature/cds sequence_feature feature/misc stop_codon feature/stop |