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BBa_J100132

BBa_J100132 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100132

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Malcolm Campbell

Date created: 2013-09-22 11:00:00

Date modified: 2015-08-31 04:08:23

E. coli thyA

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_J100132_sequence (Version 1)

Description

This is the E. coli thyA gene that converts dUMP to dTMP. Cells lacking this gene (C600thyA::KanR) need this cell to be prototrophic.

Notes

We added BioBrick prefix and suffix since the gene was synthesized by GeneArt and cloned into their pMK-T plasmid. There are no internal EcoRI, XbaI, SpeI, PstI, or BsaI restriction sites.

Source

Based on the cells used in this paper by Thompson et al., 2002. Thompson et al., 2002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Prefix Suffix BsaI cuts right sticky end BsaI cuts left sticky end start codon stop codon tyA gene	1,22 840,860 23,28 30,33 834,839 829,832 34,36 826,828 35,828	engineered_region feature/BioBrick engineered_region feature/BioBrick non_covalent_binding_site feature/binding feature/dna sequence_feature feature/binding non_covalent_binding_site feature/dna sequence_feature feature/start start_codon feature/stop stop_codon CDS feature/cds