Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Telavive Taye
Date created: 2014-12-01 12:00:00
Date modified: 2015-08-31 04:08:23
J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_J100198_sequence (Version 1) |
Description
Into the fitness gene tester construct, J100199, J100198 contains the fitness gene thymidylate synthase. J100198 contains the a theophylline riboswitch downstream of a constitutive promoter. the construct is built to be used in thymine auxotrophs that necessitate thymine for growth and division. Upon successful binding of theophylline on the riboswitch, thymidylate synthase should be expressed for the auxotrophs.Notes
N/ASource
N/A| Sequence Annotation | Location | Component / Role(s) |
| Ecor1 T5 promoter+ theophylline riboswitch thymidylate synthase LVA degradation tag stop Pst1 | 1,6 7,169 170,954 955,986 987,989 990,995 | feature/misc sequence_feature feature/dna sequence_feature CDS feature/protein feature/tag tag stop_codon feature/stop feature/misc sequence_feature |