Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Telavive Taye
Date created: 2014-09-14 11:00:00
Date modified: 2015-08-31 04:08:23
Fitness Gene Tester + LVA degradation tag
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J100199_sequence (Version 1) |
Description
We designed a fitness gene tester that allows for easy ligation of a fitness gene downstream of a theophylline riboswitch. Easy ligation is facilitated by the BsaI sites separated by a spacer sequence. The chosen fitness gene inserted into the fitness gene tester will include an LVA degradation tag that will reduce the half life of the fitness gene being tested.Notes
The fitness gene tester includes an internal BsI site and an LVA degradation tag.Source
N/A| Sequence Annotation | Location | Component / Role(s) |
| EcorI site T5 Promoter Theophylline RiboswitchD start codon BsaI site spacer BsaI site LVA d??gradation tag Stop codon PstI site | 1,6 7,58 59,164 165,167 171,176 177,197 197,202 204,236 237,239 240,245 | non_covalent_binding_site feature/binding promoter feature/promoter sequence_feature feature/dna feature/start start_codon non_covalent_binding_site feature/binding feature/misc sequence_feature feature/binding non_covalent_binding_site tag feature/tag stop_codon feature/stop non_covalent_binding_site feature/binding |