Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2014-10-12 11:00:00
Date modified: 2015-08-31 04:08:23
tClone tetA v2
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J100200_sequence (Version 1) |
Description
This device is used to test new transcriptional termination riboswitches. The default color of colonies would be green and they would be tetracycline sensitive. Users employ golden gate assembly (GGA) to remove the cassette flanked by BsaI sites and clone into this location their transcriptional termination riboswitch. In the presence or absence of ligand, the contract would become tetracycline resistant, or not, depending if the riboswitch was an on or off switch.Notes
Need to screen for internal BsaI sites. Flanked by BioBrick endings and having this synthesized as two G blocks so that we can clone this faster and cheaper than as an entire gene.Source
This was designed in the Campbell and Eckdahl labs and is related to plasmid J119137 (pClone Red).| Sequence Annotation | Location | Component / Role(s) |
| P5 (strong) promoter facing right BsaI cuts left sticky end GFP (backwards) B0030 (backwards) P2 (strong; backwards) transcriptional terminator L3S1P53 BsaI cuts right sticky end BD18, C-Dog tetA antibiotic resistance double stop | 1,36 42,47 37,40 55,774 781,795 803,848 849,898 899,904 906,909 910,995 995,2188 2183,2188 | promoter feature/promoter feature/binding non_covalent_binding_site sequence_feature feature/dna CDS feature/cds feature/rbs ribosome_entry_site feature/promoter promoter stem_loop feature/stem_loop non_covalent_binding_site feature/binding sequence_feature feature/dna feature/rbs ribosome_entry_site CDS feature/cds feature/stop stop_codon |