Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2014-12-07 12:00:00
Date modified: 2016-10-10 11:40:19
repClone Red
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_J100205_sequence (Version 1) |
Description
We designed repClone Red to test the function of repressors. The tetR repressor is present but inactive because pTet is not included. Addition of pTet will prevent translation of GFP. Addition of tetracycline to the environment (or anhydrotetracycline) will prevent inhibition by tetR. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.Notes
The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pSB1A8 with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix.Source
The two main indicator proteins are GFP (BBa_I746916) and RFP (BBa_E1010).| Sequence Annotation | Location | Component / Role(s) |
| tetR Repressor C0041 RBS BD24 P5 Promoter (for TetR) GFP RBS B0034 BsaI site P2 Promoter Terminator L3S2P21 BsaI site RBS BD18 RFP | 1,639 640,727 728,763 764,1483 1490,1501 1517,1522 1523,1568 1577,1637 1638,1643 1649,1736 1734,2414 | feature/cds CDS feature/rbs ribosome_entry_site feature/promoter promoter feature/cds CDS ribosome_entry_site feature/rbs sequence_feature feature/misc feature/promoter promoter feature/misc sequence_feature feature/misc sequence_feature feature/rbs ribosome_entry_site feature/cds CDS |