BBa_J100223

BBa_J100223 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100223

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia Preziosi

Date created: 2015-07-06 11:00:00

Date modified: 2015-08-31 04:08:24

Caffeine Aptamer with RS3, riboswitch in tCloneRed

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_J100223_sequence (Version 1)

Description

This part comes from the combination of a caffeine aptamer with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, caffeine.

Notes

We had to add the aptamer to a riboswitch so that we could get the selective termination via the stem-loop behavior. We therefore did't know how the total riboswitch would fold or if it would be a successful riboswitch.
Aptamer alone = GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGGAUCAUCGGACUUUGUCCUGUGGAGUAAGUCGCGAAACGGUGAAAGCCGUAGGUCU

Source

tCloneRed = BBa_J119361
Riboswitch 3 = J100207; Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P5 promoter	1,36	promoter, feature/promoter
BD 18 C dog RBS	182,266	feature/rbs, ribosome_entry_site
Caffeine RIboswitch	41,177	feature/stem_loop, stem_loop
RFP	267,947	CDS, feature/cds

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100223/1

Member of these Collections

• iGEM Parts Registry