Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2016-03-16 12:00:00
Date modified: 2016-03-16 02:26:18
pClone Cherry
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_J100251_sequence (Version 1) |
Description
This is a modification of pClone Red (J119137). RFP was removed and mCherry put in its place.Notes
Made sure no Bsa I sites in mCherry sequence.Source
J119137 came from the Registry. mCherry was synthesized.| Sequence Annotation | Location | Component / Role(s) |
| GFP reverse B0030 RBS reverse Sticky end BsaI left pLaclQ1 reverse BsaI right Sticky end BD18 bicistroin RBS mCherry double stop | 1,720 727,738 755,758 760,765 766,836 837,842 844,847 860,944 945,1712 1713,1718 | feature/cds CDS ribosome_entry_site feature/rbs sequence_feature feature/misc sequence_feature feature/dna promoter feature/promoter sequence_feature feature/dna sequence_feature feature/misc feature/rbs ribosome_entry_site feature/cds CDS stop_codon feature/stop |