Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Harper
Date created: 2016-04-04 11:00:00
Date modified: 2016-04-24 02:50:16
This is a modification of pClone Cherry (J100251)
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_J100253_sequence (Version 1) |
Description
This is a modification of pClone Cherry (J100251). GFP promoter pLaclQ1 was removed and a BD18 bicistroin RBS promoter was put in its placeNotes
Make sure the sticky ends aligned with the ligating promoter.Source
pClone Cherry (J100251)| Sequence Annotation | Location | Component / Role(s) |
| GFP reverse B0030 RBS reverse P5 Sticky end BD18 bicistroin RBS mCherry Sticky end | 1,720 727,738 759,794 795,798 811,895 896,1663 755,758 | CDS feature/cds ribosome_entry_site feature/rbs feature/promoter promoter feature/misc sequence_feature ribosome_entry_site feature/rbs feature/cds CDS feature/misc sequence_feature |