BBa_J100281

BBa_J100281 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100281

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hartlee Johnston

Date created: 2016-06-24 11:00:00

Date modified: 2016-07-08 12:52:04

tetA+sacB without RBS

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_J100281_sequence (Version 1)

Description

Fusion protein of tetA and sacB connected with a linker sequence, with a pBAD promotor and RBS. TetA encodes tetracycline resistance and sacB kills E. coli in the presence of sucrose, allowing for both positive and negative selection. pBAD is an arabinose-inducible promoter.

Notes

Linker sequence was modified to optimize the GC content of the primers.

Source

pBAD-RBS-tetA from Part K091133
sacB from Part J119042
linker sequence optimized from Part J119386

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pBAD	1,130	feature/promoter, promoter
tetA	137,1324	CDS, feature/protein
linker	1325,1372	feature/misc, sequence_feature
sacB	1373,2797	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100281/1

Member of these Collections

• iGEM Parts Registry