Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 01:33:06
Variant of repClone Red(J100205) w/ wt TetR promoter (R0040)
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_J100308_sequence (Version 1) |
Description
repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system. This will allow researchers and biology students to explore sequence requirements for the interactions between the TetR repressible promoter, TetR protein, and aTc.Notes
This part was constructed using Golden Gate Assembly to ligate the TetR promoter into variant repClone (J100307) following BsaI digestion.Source
Insertion of R0040 promoter in J100307 (variant repClone).| Sequence Annotation | Location | Component / Role(s) |
| tetR Repressor C0041 G->T BD24 P5 Promoter GFP Optimized T->C T->C B0034 BsaI Sticky End TetR Repressible Promoter BsaI Sticky End BD18 RFP | 1,639 243,243 640,727 728,763 764,1483 945,945 953,953 1490,1501 1512,1515 1516,1569 1570,1573 1574,1661 1659,2339 | CDS feature/protein sequence_alteration feature/mutation feature/rbs ribosome_entry_site promoter feature/promoter feature/protein CDS sequence_alteration feature/mutation sequence_alteration feature/mutation feature/rbs ribosome_entry_site sequence_feature feature/misc feature/promoter promoter feature/misc sequence_feature ribosome_entry_site feature/rbs feature/protein CDS |