Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2016-10-27 11:00:00
Date modified: 2016-10-28 03:52:41
actClone Red with wt full OmpR promoter
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J100309_sequence (Version 1) |
Description
actClone Red is a construct we designed to allow students and researchers to investigate the OmpR promoter. The RFP reporter system in actClone provides phenotypic evidence of the action of the OmpR promoter for the transcription of RFP. This construct uses the wild type OmpR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs to RFP expression using the wild-type promoter sequence (R0082). This will allow researchers and biology students to explore sequence requirements for the interactions between the OmpR promoter and phosphorylated OmpR regulatory protein.Notes
NoneSource
This part can be constructed by ligating the first 70 bp of the 5' end of the OmpR promoter (R0082) promoter into actClone (J100204) following BsaI digestion.| Sequence Annotation | Location | Component / Role(s) |
| GFP BD24 BsaI sticky end OmpR (R0082) BD21 RFP (codon optimized) | 1,720 721,805 806,809 810,917 918,1002 1003,1683 | feature/protein CDS ribosome_entry_site feature/rbs sequence_feature feature/misc promoter feature/promoter feature/rbs ribosome_entry_site feature/protein CDS |