Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2016-12-02 12:00:00
Date modified: 2016-12-02 10:53:50
repClone Red (J100205) with scrambled TetR promoter (J100311)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_J100312_sequence (Version 1) |
Description
We inserted a non-functional version of the TetR repressible promoter in repClone Red. Because the TetR binding sites have been scrambled, the promoter does not function to allow transcription of the downstream RFP, regardless of the presence of aTc. We will use this part as a negative control when using repClone Red to test the effects of modifications to the TetR promoter on its ability to promote transcription with or without aTc present.Notes
N/ASource
Assembled by inserting J100311 (scrambled TetR promoter) in repClone Red (J100205) using Golden Gate Assembly with BsaI.| Sequence Annotation | Location | Component / Role(s) |
| TetR Repressor (C0041) RBS BD24 P5 Promoter GFP RBS B0034 Scrambled TetR promoter 5' TetR binding site 3' TetR binding site RBS BD18 RFP | 1,639 640,727 728,763 764,1483 1490,1501 1516,1568 1519,1531 1544,1555 1573,1660 1658,2338 | CDS feature/protein feature/rbs ribosome_entry_site feature/promoter promoter feature/protein CDS ribosome_entry_site feature/rbs promoter feature/promoter non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site ribosome_entry_site feature/rbs CDS feature/protein |