Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2012-01-10 12:00:00
Date modified: 2015-08-31 04:08:24
Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised)
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J10063_sequence (Version 1) |
Description
The original part (K325229) was used as the template. We have removed all aspects of the pBad promoter and AraC regulatory DNA. This part now starts with the RBS in part K325229.K325229 is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence.
Notes
Wanted to allow users to use their favorite promoter.Source
from registry, synthesized de novo.| Sequence Annotation | Location | Component / Role(s) |
| endogenous RBS double stop LRE ORF Luciferin Regenerating Enzyme BamH 1 site endogenous RBS green luciferase ATG green luciferase double stop T --> C kill Spe I site | 1,17 945,950 18,20 18,950 951,956 957,973 974,976 974,2623 2618,2623 1828,1828 | feature/rbs ribosome_entry_site feature/stop stop_codon feature/start start_codon CDS feature/protein feature/dna sequence_feature ribosome_entry_site feature/rbs feature/start start_codon feature/protein CDS stop_codon feature/stop feature/s_mutation point_mutation |