pBBknock

BBa_J107077 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J107077
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michela Casanova, Lorenzo Pasotti, Susanna Zucca, Nicolo Politi, Ilaria Massaiu, Cinzia Calvio, Gabriella Cusella, Paolo Magni
Date created: 2016-01-02 12:00:00
Date modified: 2016-02-11 02:50:44

pBBknock standard vector for allelic replacement



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_J107077_sequence (Version 1)

Description

This vector can be specialized to target the desired locus in the Escherichia coli genome and carry out allelic replacement, e.g., knockout, chromosomal integration, mutagenesis, etc. See Casanova et al. (2016), Biol Proced Online for detailed instructions and protocols.

Notes

See BBa_J107112.

Source

Synthetic DNA obtained via GenScript synthesis service.

igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J107077/1