BBa_J119047

BBa_J119047 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119047

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: A. Malcolm Campbell and Todd Eckdahl

Date created: 2012-01-23 12:00:00

Date modified: 2015-08-31 04:08:26

pSB1T8

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_J119047_sequence (Version 1)

Description

Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.

Notes

Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

de novo DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Suffix	1,21	engineered_region, feature/BioBrick
Prefix	2513,2534	feature/BioBrick, engineered_region
VR primer	208,227	feature/primer_binding, primer_binding_site
VF2 primer	2375,2394	feature/primer_binding, primer_binding_site
B0025 terminator	22,205	stem_loop, feature/stem_loop
B0010 terminator	2473,2512	stem_loop, feature/stem_loop
pMB1 replication origin	314,928	sequence_feature, feature/misc
Tetracycline resistance gene	1178,2368	feature/cds, CDS
stuffer	2535,2546	sequence_feature, feature/misc
c -> g BbsI site removed	1831,1831	feature/s_mutation, point_mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119047/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors