Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: A. Malcolm Campbell and Todd Eckdahl
Date created: 2012-01-23 12:00:00
Date modified: 2015-08-31 04:08:26
pSB1T8
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_J119047_sequence (Version 1) |
Description
Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.Notes
Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.Source
de novo DNA synthesis| Sequence Annotation | Location | Component / Role(s) |
| Suffix Prefix VR primer VF2 primer B0025 terminator B0010 terminator pMB1 replication origin Tetracycline resistance gene stuffer c -> g BbsI site removed | 1,21 2513,2534 208,227 2375,2394 22,205 2473,2512 314,928 1178,2368 2535,2546 1831,1831 | engineered_region feature/BioBrick feature/BioBrick engineered_region feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site stem_loop feature/stem_loop stem_loop feature/stem_loop sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc feature/s_mutation point_mutation |