BBa_J119051

BBa_J119051 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119051

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Todd Eckdahl and A. Malcolm Campbell

Date created: 2012-01-23 12:00:00

Date modified: 2015-08-31 04:08:26

pSB4T8

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J119051_sequence (Version 1)

Description

Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.

Notes

Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

de novo DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Suffix	1,21	engineered_region, feature/BioBrick
Prefix	3935,3956	engineered_region, feature/BioBrick
stuffer	3957,3968	feature/misc, sequence_feature
VF2 primer	3797,3816	primer_binding_site, feature/primer_binding
VR	208,227	primer_binding_site, feature/primer_binding
B0025 terminator	22,205	feature/stem_loop, stem_loop
B0010 terminator	3895,3934	stem_loop, feature/stem_loop
I50042 low copy pSC101 origin	314,2350	feature/misc, sequence_feature
Tetracycline resistance gene	2600,3787	CDS, feature/cds
c -> g BbsI site removed	3252,3252	feature/s_mutation, point_mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119051/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors