Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Carr
Date created: 2012-01-25 12:00:00
Date modified: 2015-08-31 04:08:26
J23100 Promoter with BD18 RBS w/ leader and RFP reporter
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_J119053_sequence (Version 1) |
Description
The sequence contains a J23100 promoter followed by the BD18 leader RBS. The promoter has two BsaI sites, one at each end of the sequence. The one on the 5' end of J23100 cuts to the left, and the one at the 3' end of J23100 cuts to the right. There are also two BbsI sites, one is up stream from the 5' BsaI site and cuts to the left, while the other is down stream from the 3' BsaI site and cuts to the right. This is followed by an RFP gene starting with the second codon.Notes
The promoter has two BsaI sites, one at each end of the sequence. The one on the 5' end of J23100 cuts to the left, and the one at the 3' end of J23100 cuts to the right. There are also two BbsI sites, one is up stream from the 5' BsaI site and cuts to the left, while the other is down stream from the 3' BsaI site and cuts to the right.Source
de novo, selected sequence| Sequence Annotation | Location | Component / Role(s) |
| BBa_prefix BbsI Word 1 BbsI cuts left Bsa1 Word 1 J23100 BsaI Word 2 BbsI cuts right BbsI Word 2 BD18 Bicistron leader RBS GOI RBS stop for BD18 Start GOI RFP E1010 with 4 pt mutations C--> T for E. coli C--> T for E. coli C--> T for E. coli C--> T for E. coli Stop E1010 BBa_suffix | 1,22 17,20 23,28 29,32 33,67 68,71 72,77 80,83 84,171 101,109 154,162 167,169 169,171 169,849 186,186 471,471 630,630 789,789 844,849 850,870 | engineered_region feature/BioBrick sequence_feature feature/dna sequence_feature feature/misc feature/dna sequence_feature feature/promoter promoter feature/dna sequence_feature sequence_feature feature/misc sequence_feature feature/dna CDS feature/cds sequence_feature feature/misc feature/misc sequence_feature feature/stop stop_codon start_codon feature/start feature/protein CDS feature/s_mutation point_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation stop_codon feature/stop feature/BioBrick engineered_region |