BBa_J119068

BBa_J119068 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119068
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Sawyer
Date created: 2012-02-02 12:00:00
Date modified: 2015-08-31 04:08:26

RFPrev-RBSrev-pT7rev-pLacI-RBS-T7 RNAP (2SAT mutant 1)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_J119068_sequence (Version 1)

Description

RFP reporter driven by promoter pT7. T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (a??? or b) and (a or c???).

Notes

None

Source

Existing parts.

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119068/1