Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl, Claire Shinneman
Date created: 2013-04-14 11:00:00
Date modified: 2015-08-31 04:08:27
Device for Testing New Promoters via BsgI Golden Gate Assembly
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_J119141_sequence (Version 1) |
Description
This part will allow users to clone and test new promoters without gel purification or other preparation of DNA - it is ideal for teaching labs. It is a destination vector for Golden Gate Assembly (GGA) using BsgI and Ligase. A new promoter can be derived from synthetic oligos, PCR, or a plasmid clones. The new promoter must be flanked by BsgI sites that produce the 2 nt overhangs required for assembly. The left site must be 5' CTGCAC 3' and the right site must be 5' GTGCAG 3'. Successful GGA assembly replaces the reverve promoter driving RFP expression with the new promoter. The new promoter will drive RFP expression in the forward direction or GFP expression if it has reverse promoter function. The part incorporation of J23100 promoter was designed by Warsaw 2010.Notes
J119137 (Plasmid) + J23100 (Promoter)Source
Groningen 2009, Warsaw 2010, Claire Shinneman, Paul Frederick Group: iGEM2006_Davidson| Sequence Annotation | Location | Component / Role(s) |
| GFP RBS BsaI Sticky Ends BsgI J23100 BsgI BsaI Sticky Ends BD18 bicistroin RBS RFP Double Stop | 1,720 726,741 743,745 757,763 765,800 801,806 821,822 823,906 907,1614 1609,1615 | feature/protein CDS ribosome_entry_site feature/rbs feature/dna sequence_feature non_covalent_binding_site feature/binding feature/promoter promoter feature/binding non_covalent_binding_site feature/dna sequence_feature feature/rbs ribosome_entry_site feature/protein CDS stop_codon feature/stop |