BBa_J119300
BBa_J119300 Version 1 Component
This part has been discontinued.
Please consider using BBa_J100091 as a replacement.
Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2013-05-07 11:00:00
Date modified: 2015-08-31 04:08:27
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_J119300_sequence (Version 1) |
Description
This part is a modification of the J100091 part that introduces a modified, non-functional, C-Dog placeholder that when digested with BsgI will allow for insertion of a C-Dog with nested BsgI or BpmI sites. This now allows for the treatment of the C-Dog part as a swappable library tool.The design of this part is to allow the C-Dog library of parts to become "swappable" in conjunction with optimizing a metabolic network/circuit.
Notes
The C-Dog parts share a -3 NT overlap with the subsequent coding sequence, making them difficult to swap as part of a librarySource
This is a place holder part only. It has no functionality, thus upon successfully replacing this part with a functional modified C-Dog, expression of mRFP will occur.| Sequence Annotation | Location | Component / Role(s) |
| BBa Prefix BsaI sticky end left BsaI cuts left TT B0014 BsaI cuts right BsaI sticky end right BD18 bicistron leader RBS RBS for RFP Stop for BD18 leader Start for RFP RFP E1010 Double Stop for E1010 BBa suffix BBa_J100091 | 1,22 23,26 28,33 34,128 129,134 136,139 140,227 157,165 210,218 223,225 225,227 225,905 900,905 906,926 1,926 | engineered_region feature/BioBrick sequence_feature feature/dna sequence_feature feature/misc feature/stem_loop stem_loop feature/misc sequence_feature sequence_feature feature/dna sequence_feature feature/misc feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/stop stop_codon feature/start start_codon feature/protein CDS feature/stop stop_codon engineered_region feature/BioBrick feature/BioBrick engineered_region |