Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi, Jon Trocosso
Date created: 2015-06-08 11:00:00
Date modified: 2015-11-10 01:13:38
tCloneTetRed
Fusion Protein Reporter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J119386_sequence (Version 1) |
Description
This part was made from adding the TetA gene from part J119140 in front of the RFP gene in the tCloneRed part J119361. The Tet and RFP genes were combined, by deleting the "stop" sequence in the Tet gene and adding a linker sequence of proteins GGGS x4 to create a fusion protein. The resultant protein is a single protein with both the Tetracycline resistance protein and the Red fluorescent protein, combined with a linker chain. This part still contains tClone, and will not transcribe the reporter gene (Tet+Red) without the insertion of a riboswitch etc. between the BsaI sites.Notes
It was unknown whether the Tet+Red fusion protein would work as well as Tet+GFP did.Source
The part comes from J119361 tCloneRed and J119140 TetA. The linker sequence was developed from K598018 tetA+GFP fused protein by Qingyang XIAO.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter BsaI site GFP reverse P2 promoter BsaI site TetA protein half Linker Sequence RFP protein half RBS B0034 BD18 C Dog RBS | 1,36 42,47 55,774 803,848 849,854 945,2138 2139,2186 2187,2867 781,792 860,945 | feature/promoter promoter feature/binding non_covalent_binding_site CDS feature/cds feature/promoter promoter feature/binding non_covalent_binding_site feature/cds CDS CDS feature/cds feature/cds CDS feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs |