Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Athena J. Davis
Date created: 2016-06-22 11:00:00
Date modified: 2016-06-28 02:04:31
tClone TetA+SacB: Device for Testing Transciptional Terminators and Riboswitches via GGA
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J119394_sequence (Version 1) |
Description
tClone TetA+SacB can be used to clone and test new transcriptional terminators and riboswitches that function by antitermination without gel purification or other preparation of DNA. A new terminator or riboswitch can be derived from synthetic oligos, PCR, or a plasmid clone and cloned into tClone TetA with Golden Gate Assembly (GGA) using BsaI and ligase. For proper assembly, see BBa_J119367. Whether or not transcription proceeds to the TetA gene is determined by the strength of the terminator or whether termination or antitermination occurs at the riboswitch.Notes
No BsaI sites in SacB coding sequence.Source
Existing par BBa_J119374 and PCR product of SacB coding sequence.| Sequence Annotation | Location | Component / Role(s) |
| P5 BsaI Left B0030 RBS rev GFP P2 Promoter rev BsaI Right BD18 C Dog RBS TetA SacB BD18 | 1,36 42,47 781,792 55,774 803,848 849,854 861,946 946,2142 2180,3657 2148,2182 | promoter feature/promoter non_covalent_binding_site feature/binding ribosome_entry_site feature/rbs CDS feature/cds promoter feature/promoter feature/binding non_covalent_binding_site feature/rbs ribosome_entry_site CDS feature/cds CDS feature/cds ribosome_entry_site feature/rbs |