BBa_J13100

BBa_J13100 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J13100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Laura Lavery

Date created: 2005-06-21 11:00:00

Date modified: 2015-08-31 04:08:30

pOmpR dependent dual repressor device for I15030 and J13030

Details

• Types: DnaRegion
• Roles: Project; engineered_region
• Sequences: BBa_J13100_sequence (Version 1)

Description

In the presence of phosphorylated ompR the proteins CI and TetR will be coexpressed from a single mRNA. These two proteins repress the HSL and PAI amplifiers I15030 and J13030.

When using Cph1/EnvZ (I15010) as the OmpR regulator, these repressors will be expressed in the dark.

Components

Access	Instance	Definition
public	BBa_R0082	OmpR
public	BBa_B0034	BBa_B0034
public	BBa_C0051	cI lam
public	BBa_B0034	BBa_B0034
public	BBa_C0040	tetR
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0082	1,108	OmpR
BBa_B0034	117,128	BBa_B0034
BBa_C0051	135,884	cI lam
BBa_B0034	918,929	BBa_B0034
BBa_C0040	936,1595	tetR
BBa_B0010	1629,1708	BBa_B0010
BBa_B0012	1717,1757	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J13100/1

Member of these Collections

• //classic/project/uncategorized
• iGEM Parts Registry