Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-07-12 11:00:00
Date modified: 2015-08-31 04:08:32
rbs+arsR, E. coli chromosomal arsR repressor coding sequence with rbs.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_J15201_sequence (Version 1) |
Description
This is the Escherichia coli chromosomail arsR coding sequence encoding the ArsR repressor, which represses the ars promoter in the absence of arsnate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056) attached to a strong synthetic ribosome binding site BBa_J15001. Note the presence of a SacI site before the rbs, overlapping the XbaI site.Notes
Note the presence of a SacI site at the 5' end of this construct, overlapping the XbaI site. This is preserved when this biobrick is added to any other biobrick, allowing easy testing for proper insertion by restriction digestion.Source
RBS synthetic; arsR from Escherichia coli K12, sequence derived from X80057 (gi:510824).| Access | Instance | Definition |
| public public | BBa_J15001 BBa_J15101 | BBa_J15001 BBa_J15101 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J15001 BBa_J15101 | 1,10 17,373 | BBa_J15001 BBa_J15101 |