Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-07-12 11:00:00
Date modified: 2015-08-31 04:08:32
ars promoter + lacZ' + arsR
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_J15403_sequence (Version 1) |
Description
This the Escherichia coli chromosomal ars promoter, followed by lacZ' encoding the N-terminal 77 amino acids of LacZ (bet galactosidase), followed by arsR encoding the ArsR repressor which represses the promoter in the absence of arsenate or arsenite (see Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056). Its function is to generate LacZ activity in the presence of arsenate or arsenite. Note that this requires a host strain such as E. coli JM109, with the lacZ-delta-M15 mutation, providing the C-terminal part of LacZ.Notes
No issuesSource
The ars promoter and arsR are from E. coli K12 (gi: 510824) and lacZ' from E. coli BS (gi: 146575).| Access | Instance | Definition |
| public public public public public | BBa_J15301 BBa_J15001 BBa_J15102 BBa_J15001 BBa_J15101 | BBa_J15301 BBa_J15001 BBa_J15102 BBa_J15001 BBa_J15101 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J15301 BBa_J15001 BBa_J15102 BBa_J15001 BBa_J15101 | 1,127 136,145 152,385 394,403 410,766 | BBa_J15301 BBa_J15001 BBa_J15102 BBa_J15001 BBa_J15101 |