Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brady Laughlin
Date created: 2011-10-22 11:00:00
Date modified: 2015-08-31 04:08:35
V0201
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_J176126_sequence (Version 1) |
Description
VP64-NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neoNotes
TBASource
TBA| Sequence Annotation | Location | Component / Role(s) |
| V5 epitope tagging BGH reverse priming site BGH polyadenylation signal FRT site Hygromycin resistance gene SV40 early polyadelylation signal pUC origin bla (ampicilin resistance) bla promoter Digestion product of ApaI and HindIII building bloc variation Digestion product of ApaI and HindIII building bloc variation digestion overhangs stop codon? micro MCS site for biobrick inclusion SpeI - XbaI ligation SpeI - XbaI ligation 3t3 forward primer bind 3t3 forward primer 2.0 hopefully better possible biobrickable terminator with polyA signal terminator possible biobrickable terminator with polyA signal terminator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator bglobinsulator | 1066,1107 1145,1162 1151,1375 1658,1695 1713,2705 2865,2995 3378,4051 4196,5056 5057,5155 1020,5157 1,4 1051,1054 1129,1131 1020,1050 1045,1047 1048,1048 906,930 920,943 1117,1375 763,767 331,331 65,404 321,340 321,340 390,404 411,750 411,425 405,410 677,677 667,686 667,686 736,750 52,54 47,51 760,762 60,74 64,64 1,5157 | feature/misc sequence_feature feature/misc sequence_feature feature/polya polyA_site sequence_feature feature/misc CDS feature/cds feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS feature/promoter promoter feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/cds CDS primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/cds CDS feature/primer_binding primer_binding_site feature/misc sequence_feature feature/misc sequence_feature primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/primer_binding primer_binding_site feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site feature/misc sequence_feature |